An Introduction to Advanced Systematic Enzyme Technology

INTRODUCTION

Dinazyme B/W Liquid is a liquid acid-neutral xylanase/b-glucanase/pectinase/cellulase product from an advanced non-GMO non-pathogenic strain of Trichoderma longibrachiatum (formerly T. reesei) producing enzymes Generally Recognized As Safe for food applications (FDA Ruling Docket No. 79G-0372), also on the accepted list of AAFCO (see Official Publication, 1999, pp. 208-209, and 2000, p. 226) and Canadian list of approved protein feeds (entries 5.4.42 and 5.4.43).

Products of this origin are used world-wide as processing aids in baking, juice extraction, wine clarification and animal feed. Its levels of xylanase / b-glucanase / pectinase /cellulase activities allow the enzyme to break down arabino-xylans, b-glucans and other non-starch polysaccharides in poultry, swine and aquaculture diets, containing high amounts of wheat, rye, barley, triticale and certain other cereals. It should be noted, that without such enzymes the broiler diets cannot accommodate more than 20% wheat or 15% barley or 15% rye.

Why include Dinazime B/W Liquid ® in your rations ?

• For better feed utilization when using feedstuffs containing high levels of arabanoxylan and beta-glucan such as wheat and rye.
• To maximize animal performance, even when digestion capacity is limited e.g. in times of stress.
• To upgrade lower quality feedstuffs, enabling least cost formulation.
• To reduce or eliminate wet litter problems caused by high digesta viscosity.
• For a stable and safe enzyme option.
• To improve litter quality by reducing digesta viscosity.

PHYSICAL PROPERTIES

According to IUPAC definition, 1 IU of enzyme activity is providing the formation of 1 micromole/min (6.03 *1017/min) of cleavages of polysaccharide chains (resulting in generation of the amount of reducing ends chemically equivalent to 0.18 mg of Xylose/min).

PRODUCT CAPABILITIES

1. Decrease viscosity of arabinoxylans, b-glucans and pectins in high-wheat and
   high-barley poultry diets to minimize wet droppings.
2. Break down cell wall polysaccharides to increase energy utilization from
   high-wheat and high-barley poultry, swine and aquaculture diets.

PROCESSING CONDITIONS: GENERAL

Dinazyme B/W Liquid is designed to be included into poultry and swine diets at a rate of 200 grams per ton of feed. Additional dilution prior to application is possible, the degree of dilution being based of the capabilities of the pumping equipment to accurately dose and distribute the liquid enzyme product through the volume of feed. However, the product diluted with water should not be stored for more than one week at temperature exceeding 32°C (90°F). Please, contact Diversified Nutri-Agri Technologies if more stable diluted formulations are desirable.

When included into the final poultry and swine diets, the enzyme can undergo heating during pelletizing the feed at temperature not exceeding 85°C (185°F) for several minutes. However, for best results, spray-on after pelletizing is recommended to minimize the activity loss. Product addition into the drinking water is another possibility.

The optimal action of Dinazyme B/W Liquidis displayed when the product is included into poultry and swine diets and consumed by animals into the digestive tract. The enzyme is stable under acid stomach conditions and starts performing in the intestine. In analytical tests activity is displayed at temperature of 40EC - 57EC (100EF - 135EF) and pH of 4,2 - 6.5.

Diversified Nutri-Agri Technologies , Inc. is constantly testing their enzyme products in customer-oriented applications providing the most adequate technical and application support of the enzyme use.

STORAGE CONDITIONS/ACTIVITY

Dinazyme B/W Liquid will retain more than 90% of declared activity after four months when stored at 23°C - 25°C (75°F - 77°F).

Dinazyme B/W Liquid will also retain more than 90% of declared activity after 1 month when stored at 37°C (100°F) out of direct sunlight and in the original, closed container. Do not allow Dinazyme B/W Liquid to freeze.

TECHNICAL SERVICE

Information covering specific applications for this product is available from your Diversified Nutri-Agri Technologies sales/technical representative. We will work with your technical personnel to resolve problems and optimize your process.

SAFETY AND HANDLING

For detailed information please refer to the Dinazyme B/W Liquid MSDS available upon request. We also recommend consulting the Enzyme Technical Association handbook, Working Safely with Enzymes, or the various AMFEP publications on the safe handling of enzymes.

Nothing disclosed is to be construed as a recommendation to use our products in violation of any patents. The information presented is believed to be accurate.

Illustration depicts a plausible fit model for Dinazyme/substrate interactions.
The most precise fit occurs during transition state that precedes the final reaction.

Normal effect of pH on enzyme activity. Dinazime B/W Liquid ® is able to withstand
a wide range of pH ranges while maintaining efficacy. The colored lines depict
maximum functionality for normal pH dependant enzymes. Purple is for acidic,
brown for neutral, and red for basic solutions.

What diets are best suited to the use of Dinazime B/W Liquid ® ?

Activity over a wide pH and temperature range means that Dinazime B/W Liquid ® is suited to a variety of animals and feeding conditions. Dinazime B/W Liquid ® can be used in the feeding programs for any species, although the greatest results are obtained when included in monogastric diets.

PRODUCT SPECIFICATIONS

Physical Characteristics

Storage and Stability

Dinazime B/W Liquid ® is non-toxic and non-pathogenic. Store in a dry location with temperature not exceeding 80°F. The stability during storage depends on storage temperature. Under normal storage conditions (70-80°F), the activity of Dinazime B/W Liquid ® can be guaranteed to remain within product specifications for at least 4 months.

Packaging